ELISA: Mycobacterium avium subsp. paratuberculosis Antibody (6G11) [NBP2-54646] - Bacterial suspension of irradiated MAP was added wells as 'Free MAP cells' prior to the addition of the antibody. Negative control wells received assay buffer followed by the antibody. A decrease in absorbance values in the presence of 'free MAP cells' over negative control wells is indicative of MAP binding. Figure depicts the mean absorbance values (n = 3) standard error. Mean % binding inhibition was calculated based on the difference in the % binding of the antibody to the attached MAP cells in the presence and absence of 'free MAP cells', where negative control wells represent 100% binding.